Journal: eLife

Article Title: Syntaxin11 deficiency inhibits CRAC channel priming to suppress cytotoxicity and gene expression in T lymphocytes independent of membrane trafficking

doi: 10.7554/eLife.111335

Figure Lengend Snippet: ( A–C ) Fura-2 calcium imaging assay measuring SOCE in cells treated with Scr shRNA or different shRNA sequences against SNAP23 ( A ) SNAP25 ( B ) SNAP29 ( C ). Average traces from 30 to 40 cells per group are shown. ( D ) Western blot images of co-IPs to test the association of Orai1 with SNAP23/SNAP25/SNAP29. HEK293 cells were co-transfected with Orai1-Myc and untagged SNAP23/SNAP25/SNAP29, store-depleted, lysed, and subjected to co-IP followed by western blot as indicated. (N=2) ( E ) Western blot images of co-IP to test the association of Stim1 with SNAP23/SNAP25/SNAP29. HEK293 cells were co-transfected with YFP-Stim1 and Myc-tagged SNAP23/SNAP25/SNAP29, store-depleted, lysed, and subjected to co-IP followed by western blot as indicated. (N=2). Figure 2—figure supplement 3—source data 1. Numerical data underlying the quantitative analyses represented in . Figure 2—figure supplement 3—source data 2. Folder containing labeled PDF files of the original western blots for . Figure 2—figure supplement 3—source data 3. Folder containing the original images of western blots shown in .

Article Snippet: Antibody , Rabbit polyclonal anti-SNAP23 , SYSY , 111203 , WB (1:1500).

Techniques: Imaging, shRNA, Western Blot, Transfection, Co-Immunoprecipitation Assay, Labeling

Journal: eLife

Article Title: Syntaxin11 deficiency inhibits CRAC channel priming to suppress cytotoxicity and gene expression in T lymphocytes independent of membrane trafficking

doi: 10.7554/eLife.111335

Figure Lengend Snippet:

Article Snippet: Antibody , Rabbit polyclonal anti-SNAP23 , SYSY , 111203 , WB (1:1500).

Techniques: